System Highlight

• Sterility: An exclusive patented microsyringe technology allows for genuine sampling sterility. Since no disinfectants are used, Eddy Jet 2 does away with any possible false negative results due to disinfectant carryover.
• Simplicity: Get trained in less than 5 minutes: insert a Petri plate, press on the “play button” in Eddy Jet 2’s innovative color touch screen, and that’s it, PLUG & PLATE!
• Fully Validated: Spiral plating is a fully validated Microbiological method, see: ISO 7218/ AOAC 977.27/ FDA (BAM Ch.3). Verification protocols are available for each section of the device’s spirals.
• Advantageous Mechanics: Eddy Jet’s dependable Stepper Microcontroller motors regulate the instrument’s high precision microsyringe liquid dispensing. This approach avoids all the issues of competing technologies where cleaning cycles, disinfectant preparation, cross contamination, unnecessary and complex liquid handling, stylus calibration, noisy vacuum pumps, and complicated maintenance requirements soon shadow the advantages of spiral plating.
• Connectivity: Eddy Jet 2 features cutting edge connectivity; it can be connected to a barcode reader and a printer in order to provide plate traceability. It’s USB and Ethernet connections allow for connection of a key board for data insertion or a pendrive for upgrades.

Aplication scope

Many samples can be processed in Eddy Jet 2; aliquots taken from solutions of any diluted sample are placed inside specially devised and introduced inside Eddy Jet 2 for processing. During dispensation allow the device to perform a wide range of up to 34 types of spirals. Eddy Jet 2 inoculates 90 and 150 mm plates alike thanks to its special . Using 150 mm plates allows to generate an even larger concentration gradient having a single plate account for almost 5 log10 concentration range.

Spiral plating is most frequently used for enumeration of samples but several other applications exist. Thanks to the lawn spiral distribution Eddy Jet 2 can be used to create bacterial lawns meant to be used in AST for Kirby-Bauer testing. Spiral gradient testing allows to test susceptibilities of multiple strains to a single antibiotic. The Spiral Salmonella assay for mutagenicity testing, a spiral plating equivalent of the AMES test, can also be performed using Eddy Jet 2. Eddy Jet 2 linear modes enable to refine the detection of mutagenic concentration thresholds.

How to continue procesing diluted samples

Initially samples are suspended in a diluent broth through gravimetric dilutions performed in either the Smart Dilutor W, sample-containing bags can be stored in Portabag bagholders, awaiting to be homogenized in Masticator blenders. When homogenizing samples, filter bags are to be used for debris generating samples while for other samples filterless bags shall suffice. Once homogenized, aliquots can be serially diluted using the . The Eddy Jet 2 spiral plater is used in order to reduce the amount of plates that are plated, generating a threefold decimal dilution counting range in a single plate. Following incubation, plates are automatically counted and fed with the Plate Handler or automatically counted and manually fed with the SphereFlash®.

Technical Specification

Videos:

https://youtu.be/nUT2oCHvYto